Supplementary Figure 5

(a) Western blot analysis of Kank2 and Liprin-β1 in wild-type cells expressing FL-Kank2-GFP together with scrambled (shScr) and two Liprin-β1-specific shRNAs. Vinculin served to control protein loading. (b) 2D correlation coefficient between GFP and inverted FRET signals in FL-Kank2-GFP expressing cells before and after Liprin-β1 depletion (dot plot and box plot; FL-Kank2 shScr, n = 30 cells; FL-Kank2 shLiprin-β1#1, n = 31 cells; FL-Kank2 shLiprin-β1#2, n = 33 cells; data aggregated from 3 independent experiments for each condition; P values were calculated using the Wilcoxon Rank Sum test). (c) Ratio between adhesion area with high tension (≥250 pN μm⁻²) and adhesion area with low tension (<250 pN μm⁻²) were calculated in cells expressing indicated constructs (dot plot and box plot; FL-Kank2, n = 24 cells; Kank2ΔCoil, n = 45 cells; FL-Kank2 shScr, n = 30 cells; FL-Kank2 shLiprin-β1#1, n = 31 cells; FL-Kank2 shLiprin-β1#2, n = 33 cells; data aggregated from 3 independent experiments for each condition; P values were calculated using the Wilcoxon Rank Sum test).