Supplementary Figure 6 | Nature Cell Biology

Supplementary Figure 6

From: Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation

Supplementary Figure 6

(a) SDS-PAGE analysis of bacterially expressed and purified FL-Talin-1, Talin head domain (THD), Venus-His-Sumo-tagged Talin R4-R8 domain, GST-KN fusion protein and GST. (b) HEK293 cells expressing GFP-tagged Talin rod truncations were lysed, incubated with recombinant GST-KN, immunoprecipitated with anti-GFP antibody and analyzed with Western blot. Red asterisk marks the recombinant GST-KN protein.

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