Supplementary Figure 1

(a) SILAC ratio plot showing specific β1wt tail interactors with high β1 wt/β1 scr and low β1 scr/β1 wt SILAC ratios in reverse SILAC labeling experiments and with high MS intensities in the FA-enriched proteome of FN-seeded fibroblasts in red. Dab-2 (green) and SNX17 (grey) show a high β1 wt/β1 scr SILAC ration but were absent from the FA-enriched proteome. (b) Split channels of Fig. 1b. Mouse fibroblasts seeded on FN for 3h and immunostained for Kank2 (green), Kindlin-2 (red) and DAPI (blue). (c) Mouse fibroblasts seeded on FN for 3h and immunostained for Kank2 (green), Talin (red) and DAPI (blue). (d) Confocal image of immunostained (Kank2, green; Kindlin-2, red; DAPI, blue) wild-type fibroblasts seeded for 3h on a FN-coated micropattern. The boxed areas show Kank2 puncta around paxillin-positive FAs. (e) Mouse fibroblasts seeded on FN for 5h and immunostained for β1 integrin (Itgb1, green), β3 integrin (Itgb3, red), Paxillin (Pxn, blue) and DAPI (grey). (f,g) Mouse fibroblasts expressing Kank2-GFP seeded for 5h on FN and immunostained for β1 integrin (Itgb1, red) or active β1 integrin (9EG7, red), Paxillin (Pxn, blue) and DAPI (grey). Scale bar in b-g, 10 μm.