Supplementary Figure 4: Effect of PP2A inhibition on endothelial cell adhesion.
From: Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling
(a) BAECs were transfected with PP2A siRNA or treated with okadaic acid (OA, 5 nM, 1hr) and plated on FN (10 μg/ml) for 1hr. Cell adhesion was quantified as described in Supplementary Fig. 2 (n = 3 independent experiments) and cell morphology was examined after fixation and phalloidin and nuclear staining. Error bars are SEM. Scale bar: 100 μm. (b) BAECs treated as (a) were plated on FN (10 μg/ml) for indicated times and FAK phosphorylation was measured by Western blotting.
