Supplementary Figure 5: In vivo knock-down of endothelial PDE4D.
From: Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling
(a) NIH3T3 cells were transfected with either luciferase siRNA or mouse PDE4D siRNA used for in vivo knock-down. After 72h, PDE4D5 was assayed by Western blotting with tubulin as a loading control. (b) Nano-particles containing PDE4D siRNA or luciferase siRNA (1 mg/kg) were injected intravenously. After two weeks, mouse aortas were isolated, and endothelial expression of PDE4D was assayed by qPCR (n = 4). (c) Serum lipid profile of α5/2; ApoE(-/-) mice after high fat diet (n = 6 mice). Data are represented as means ± SEM. ∗p < 0.05 by two-tailed t-test. Source data are provided in Supplementary Table 1.
