Supplementary Figure 2: PDE4D5 is responsible for ECM-dependent inflammation in endothelial cells.
From: Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling
(a) BAECs on FN or collagen were treated with rolipram (1 μM) and assayed for AMPK activation. (b) Immunoblotting HUVEC lysate with pan PDE4D antibody detects the major band co-migrating with reference PDE4D5 (left panel). The band was diminished by transfection with PDE4D siRNA, demonstrating specificity. Similarly, PDE4D5 was the only isoform detectable in BAEC lysate with immunobotting using pan PDE4D antibody or PDE4D5 specific antibody (right two panels). ∗ indicates non-specific bands. (c) BAECs expressing wild type PDE4D5 or FAT-PDE4D5 were plated on MG for 1hr to monitor GFP signal.
