Supplementary Figure 2: Microtubules self-repair at photo damage sites.

Microtubules were grown in the device shown in Fig. 4a. Green microtubule seeds were elongated with red free tubulin (step I). Photo damage (yellow star) was induced in the presence of green tubulin (step II). After a two-minute delay, the medium was changed again for red tubulin (step IV). Free fluorescent tubulin dimers were removed and replaced with non-fluorescent dimers to visualize microtubules. The white arrow point at the incorporation of green tubulin along the pre-existing red microtubule following laser-induced damage.