Supplementary Figure 5: EB1 recruitment at microtubule repair sites.

(a) EB3 recruitment at repair sites in the presence of free tubulin. Red microtubules were photodamaged in the presence of red-fluorescent free tubulin dimers and EB3-GFP. Images show microtubule fluorescence in the red (left) and green (right) channels, before (top) and after (bottom) the laser-induced damage. The graph shows fluorescent linescans along this microtubule before and after the damage. The yellow star represents the damaged site. (b) Absence of EB3 recruitment at repair sites in the absence of free tubulin. Same experiment in the absence of free tubulin. (c) Quantification of the effect of laser damage on the ratio of EB3 intensity after/before.