Supplementary Figure 6: Laser photo damage promotes microtubule growth.

(a) Time lapse and kymograph of a microtubule in vivo with multiple photo-damage sites. Microtubule was photo-damaged (yellow stars) close to the tip, promoting multiple rescue events (red arrowhead). Though catastrophe events were frequent, this microtubule was protected from complete depolymerization by the photo damage. Scale bar is 5 μm. (b) Time lapse of a growing microtubule in vitro without or with laser damage. Left, a growing microtubules in absence of laser damage. Right, a growing microtubule in presence of repeated laser damages. Image sequences corresponding to the kymographs shown in Fig. 6a.