Supplementary Figure 1: SCRIB is required for reactive oxygen species (ROS) generation.

(a) Schematic diagram of the tetracycline-inducible knock-in constructs used to generate SCRIB shRNA mice. (b) Bone marrow derived macrophages were imaged with confocal microscope. Representative images are shown. Scale bar represents 50 μm.. Bone marrow cells were isolated from WT + Dox or − Dox, ishSCRIB + Dox or − Dox mice, and then neutrophils were isolated. Cells were then stained for flow cytometry and analyzed by flow cytometry checking the purity of neutrophils (c), or the expression of ishSCRIB (GFP) (d). (e) Immunoblot analysis of PDGF (50 ng ml⁻¹) induced PDGFR-β tyrosine phosphorylation in MEFs at indicated time points. Immune cells isolated from bone marrow, spleen, thymus from WT and ishSCRIB mice (8 to 12 weeks old) with normal food or Dox food were analyzed GFP signal (f), Splenic cells stained with indicated surface marker for dendritic cells, neutrophils, and monocytes (g); bone marrow cells stained with surface marker for neutrophils, and monocytes (h); splenic cells and thymic cells were stained with T cell surface maker (i); bone marrow cells stained with B cell surface marker (j); and splenic cells stained with B cell surface maker (k).