Supplementary Figure 3: Phenotypes of dorsal closure in ES-SqhKO embryos.
(a) mCherry-moesin (labels F-actin) expressing control and ES-SqhKO embryos at the end of dorsal closure. 8% of the embryos show no puckering defects (n = 89), 65% show mild puckering defects (red arrows) and 27% show a severe puckering defect. Scale bar: 50 μm. Images are representative of 89 embryos from >5 experiments. (b) mCherry-moesin (labels F-actin) expressing control and ES-SqhKO embryos at the end of dorsal closure. In 41% of the embryos (n = 95) the anterior epidermis ruptures. Scale bar: 50 μm. Images are representative of 95 embryos from >5 experiments. (c) Recoil of epidermis (Sqh-GFP labelled) after laser incision in control and ES-SqhKO embryos. Red arrows indicate the extent of tissue recoil, black arrows show the direction of the laser cut. Scale bar: 20 μm. Images are representative of 4 control and 3 ES-SqhKO laser incisions/embryos from 1 experiment. (d) Gap width change in control (black, n = 19 embryos, pooled from 3 independent experiments) and ES-SqhKO embryos (red, n = 26 embryos, pooled from 6 independent experiments). Dashed lines: Standard deviation. Mann-Whitney U test: ∗P < 0.05. (e) Convergence speeds of the two lateral LEs in control (black, 19 embryos, pooled from 3 independent experiments) and ES-SqhKO embryos (red, 26 embryos, pooled from 6 independent experiments). The right panel is an overlay of the first two panels. Statistics source data for d and e is provided in Supplementary Table 2.
