Supplementary Figure 1: Selective Sqh-GFP depletion in amnioserosa cells. | Nature Cell Biology

Supplementary Figure 1: Selective Sqh-GFP depletion in amnioserosa cells.

From: Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure

Supplementary Figure 1

(a) UAS-mCherry-moesin expression pattern of the most commonly used amnioserosa-specific Gal4 enhancer elements 332.3Gal4 and c381Gal4. Arm-GFP outlines the amnioserosa tissue in the embryos with leaky c381Gal4 expression. Scale bar: 50 μm. (b) Selected movie frames showing dorsal closure progression in AS-SqhKO embryos, expressing rescuing Sqh-mCherry. Left column shows Sqh-GFP signal, right column show Sqh-mCherry signal. Scale bar: 50 μm. Images in a and b are representative of >10 embryos from >2 experiments. (c) Sqh-GFP-expressing embryos, selectively expressing UAS-Mbs.N300 in the amnioserosa tissue. Scale bar: 20 μm. Image represents one experiment with 4 embryos. (d) Relative gap area change (%/min) in control (black, n = 19 embryos, pooled from 3 independent experiments) and AS-SqhKO embryos (red, n = 23 embryos, pooled from 4 independent experiments). Mann-Whitney U test: ∗P < 0.05, ∗∗∗P < 0.001. Dashed lines: Standard deviation. (e) Convergence speeds of the two lateral LEs in control (black, 19 embryos, pooled from 3 independent experiments) and AS-SqhKO embryos (red, 23 embryos, pooled from 4 independent experiments). Dashed lines: Standard deviation. (f) Gap length changes in control (black, n = 19 embryos, pooled from 3 independent experiments) and AS-SqhKO embryos (red, n = 23 embryos, pooled from 4 independent experiments). Mann-Whitney U test: ∗P < 0.05, ∗∗∗P < 0.001. Dashed lines: Standard deviation. Statistics source data for d and f is provided in Supplementary Table 2.

Back to article page