Supplementary Figure 2: Sqh-GFP depletion in epidermis cells with pnr enhancer element. | Nature Cell Biology

Supplementary Figure 2: Sqh-GFP depletion in epidermis cells with pnr enhancer element.

From: Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure

Supplementary Figure 2

(a) Selected movie frames showing germ band retraction and dorsal closure failure in pnrES-SqhKO embryos, with the leaky pnr enhancer element. The dashed red line represents the LE. Cartoon: In green are cells with functional Sqh-GFP. Scale bar: 50 μm. (b) 3 examples of embryos expressing deGradFP with the leaky pnr enhancer element. One example each of an affected, expanding amnioserosa cell and an unaffected, constricting amnioserosa cell is outlined in red and blue respectively. Scale bar: 20 μm. (c) Schematic showing strategy for Gal4 swapping with Gal80 in the 332.3 locus. (d) Embryos in which the leaky pnr enhancer element was used to drive expression of the actin-marking mCherry-moesin without (top) and in combination with 332.3Gal80 (bottom). Red arrows indicate amnioserosa cells with ectopic mCherry-moesin expression by pnr. Scale bar: 10 μm. Images in a,b and d are representative of >10 embryos from >3 experiments.

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