Supplementary Figure 1: Systematic profiling of DSB-containing chromatin by CHROMASS.

(a) Etaa1 sequence coverage in CHROMASS experiments. Identified peptides are indicated by red bars above the amino acid sequence. (b) Profile plots for the recruitment of Etaa1 and other indicated factors to DSB-containing chromatin at different time points. The z-scored log2 LFQ intensity (mean of three replicates) was plotted against time. Note that the recruitment profile of Etaa1 closely follows that of the RPA complex (RPA1-3). (c) Cluster analysis. The mean averaged intensity profile of RPA (Rfa1, Rfa2 and Rfa3, shown in dark green in Supplementary Fig. 1b) was used to calculate distances for proteins (2668 in total) that had at least 50% valid values. Proteins were ranked according to increasing distances. Analysis was done with the Profile Plot function implemented in Perseus. (d) Undamaged chromatin was incubated in HSS Xenopus egg extract to license chromatin for replication. DNA replication was initiated by addition of NPE Xenopus egg extract. Aphidicolin was added to NPE to inhibit DNA polymerases, thereby triggering uncoupling of replicative helicase and polymerase movements (see right panel). The volcano plot shows the mean difference of the protein intensity relative to reactions containing the replication inhibitor geminin plotted against the P value. The graph was replotted from primary data described in³¹.