Supplementary Figure 4: ERj3 depends on Sec62:Sec63 for post-translational translocation in the mammalian ER.
From: Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery
In a complementation or rescue assay, the Sec62 and Sec62LIRm equally support ERj3 translocation into the ER of Sec62 silenced HeLa cells (please also refer to Fig. 6f for KO cells generated with the CRISPR-Cas9 gene editing technology). Expression (Sec62) and transport (ERj3) efficiencies were calculated as described in the Methods section. n = 4 independent experiments, mean ± SEM. We note that SEC62 plasmids (lanes 2–4 and 6–8) lack the untranslated region (UTR) of endogenous mRNA and, therefore, are resistant to UTR-targeting siRNA.
