Supplementary Figure 6: Separation of AV and ER in isopycnic density gradients.
From: Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery
(a) Schematic representation of the experiments. At the end of the ultracentrifugation, AV float at the top of the density gradient, the ER has higher density (Fig. 8a). (b) Extracts of WT and Atg7 KO MEF recovering from ER stress were prepared and loaded at the bottom of an isopycnic density gradient (as in Fig. 8a and Methods). Fractions floating at the top of the gradient (AV) and fractions containing the ER were separated in SDS-PAGE and probed for the presence of Cnx, Sec62 and LC3 by WB. WB representative of 2 independent experiments.
