Supplementary Figure 6: Hsp40 (DNAJA1), but not Hsp90, is involved in statin-induced mutp53 degradation, and reduced MVP by statins changes the binding partner of mutp53 from DNAJA1 to CHIP.
From: DNAJA1 controls the fate of misfolded mutant p53 through the mevalonate pathway
(a) WB for Hsp90, Hsp90 client proteins (p53R175H, EGFR, Raf-1, and ErbB2), and β-actin or GAPDH using SK-Br-3 cells treated with DMSO (D) or lovastatin (4 μM). (b) Immunofluorescence for p53R175H using SK-Br-3 cells infected with lentiviral vectors encoding control or DNAJA1 shRNAs and treated with vehicle (ethanol) or leptomycin B (LMB, 50 nM). Scale bar, 50 μm.
