Supplementary Figure 7: Reduced MVP inhibits mutp53’s binding to DNAJA1 but increases its interaction with CHIP.
From: DNAJA1 controls the fate of misfolded mutant p53 through the mevalonate pathway
(a) Co-immunoprecipitation and WB for p53R175H and DNAJA1 using CAL33 cells treated with D or L for 11 h, before the degradation of mutp53. (b) Co-immunoprecipitation and WB for p53Y 220C and DNAJA1 using BxPC-3 cells treated with D, L, or L + MVP for 11 h. (c) Co-immunoprecipitation and WB for p53Y 220C and CHIP using BxPC-3 cells treated with D or L for 11 h. (d) A schematic view of a proposed mechanism for statin-induced mutp53 degradation. In the absence of DNAJA1, conformational/misfolded mutp53 is degraded by CHIP, suggesting that DNAJA1 determines the fate of mutp53.
