Supplementary Figure 4: Analysis of Lgr6-expressing cells in the adult mammary gland. | Nature Cell Biology

Supplementary Figure 4: Analysis of Lgr6-expressing cells in the adult mammary gland.

From: Lgr6 labels a rare population of mammary gland progenitor cells that are able to originate luminal mammary tumours

Supplementary Figure 4

(a) Flow cytometry dot plot showing distribution of EGFP expression in MECs of 8w-old Lgr6-CreERT2+/− females. (b) Bar graph demonstrating the recombination efficiency 24 h after tamoxifen administration to 8w-old (n = 4 mice) Lgr6-CreERT2+/−:Rosa26-tdTomato+/− (LT) females. Mean ± s.e.m. (c) Flow cytometry plot showing the distribution of tdTomato-labelled cells over MECs 24 h p.i. (d) Flow cytometry dot plots of primary MECs 22w p.i., showing the distribution of tdTomato-labelled cells. (e) Representative flow cytometry plots showing very rare EGFP+ cells (left panel), present in both MEC compartments (right panel) of mammary glands from 30w-old Lgr6-CreERT2+/− females. (f) Confocal z-stack image (image 1) of K14/K8 immunostaining showing adjacent basal and luminal tdTomato+ clones (arrowheads) in 1d L1 alveoli of 8w-induced LT mice. Images 2–11 show the resolution of the 3D image into 10 single plane images. Insets show magnified regions containing adjacent basal and luminal tdTomato+ cells (arrowheads). Scale bar, 20 μm. (g) 3D reconstruction of duct at 14.5 dpc showing multi-cellular tdTomato+ cell patches containing K14+ (arrows) and K8+ cells (arrowheads). Scale bar, 50 μm.

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