Supplementary Figure 5: Activation of oncogenic Ras combined with Fbxw7 deletion in Lgr6-positive cells results in breast cancer. | Nature Cell Biology

Supplementary Figure 5: Activation of oncogenic Ras combined with Fbxw7 deletion in Lgr6-positive cells results in breast cancer.

From: Lgr6 labels a rare population of mammary gland progenitor cells that are able to originate luminal mammary tumours

Supplementary Figure 5

(a) Description of the FRL mouse strain, derived from crossing three strains (Fbxw7f/f; LSL-KRasG12D; Lgr6-CreERT2). (b) Treatment scheme of 5-week-old FRL mice with tamoxifen and subsequent tumour analysis. (c) Immunohistochemistry of tumours from FRL mice 3 weeks post-injection with tamoxifen, stained with haematoxylin-eosin (HE) and probed for markers against basal (K5) and luminal (K8) cells. Scale bars, 100 μm. (d) Immunohistochemistry of tumours from FRL mice 8 weeks post-tamoxifen injection stained with HE and probed for K5, K8 and ERα. Scale bars, 100 μm. (e) Summary of BPL and FRL tumour lesions.

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