Supplementary Figure 5: Evaluation of clustering quality for small clones.
From: Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development
(a) Tg(drl:creERT2;ubi:Zebrabow-M) embryos were treated at 96 hpf, and their marrow was analyzed by flow cytometry at 5 months to measure small clonal contributions. (b) Silhouette plots from three representative marrows evaluate the quality of the clustering solutions. Small clones that contribute <1% of the granulocyte population were detected with high silhouette values, demonstrating the low threshold for detection in this system.
