Supplementary Figure 5: Characterization of MAYA-LLGL2 and MAYA-NSUN6 interactions.
From: A ROR1–HER3–lncRNA signalling axis modulates the Hippo–YAP pathway to regulate bone metastasis
(a) Electrophoresis of in vitro transcribed Xef1α (left) or MAYA (right) sense (sen.) and anti-sense (as.) transcripts. (b) Streptavidin RNA pull-down assay, followed by IB detection of indicated proteins, using MAYA sense (sen.), anti-sense (as.) and Xef1α sense RNA. The presence of RNA transcripts was detected by streptavidin-HRP using dot-blot assay. (c) Electrophoresis of in vitro transcribed full-length MAYA or truncated transcripts. (d) RNA REMSA assay was performed using recombinant GST-tagged LLGL2 (aa. 101-200) or FLAG-tagged NSUN6 in the presence of 32P-labeled or cold MAYA RNA probes (nt. 251-290 and nt. 851-890), respectively. (e) Cell fractionation followed by RT-qPCR detecting relative MAYA expression in MDA-MB-231 cells with indicated stimuli (mean ± s.e.m. were derived from n = 3 independent experiments, n.s., P > 0.05, two-tailed paired Student’s t-test). Unprocessed original scans of all blots/gels with size marker are shown in Supplementary Fig. 9. An unprocessed original scans of EMSA image for d is also shown in Supplementary Fig. 9.
