Supplementary Figure 6: Examination of the role of LLGL2 and NSUN6 in breast cancer cells.
From: A ROR1–HER3–lncRNA signalling axis modulates the Hippo–YAP pathway to regulate bone metastasis
(a and b) IB detection of the indicated proteins in LLGL2 KO (a) and NSUN6 KO (b) MDA-MB-231 cells. (c and d) Cell proliferation assay of individual (c) and pooled (d) clones of LLGL2 KO (left panels) and NSUN6 KO (right panels) MDA-MB-231 cells. (e and f) Cell invasion assay of individual clones of LLGL2 KO (e) and NSUN6 KO (f) MDA-MB-231 cells with overexpression of indicated plasmid (left, Scale bars, 200 μm; right, quantification). (g and h) Cell migration (g) and invasion (h) assay of pooled clones of LLGL2 KO and NSUN6 KO MDA-MB-231 cells (left, Scale bars, 200 μm; right, quantification). For c–h, mean ± s.e.m. were derived from n = 3 independent experiments (n.s., P > 0.05, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001, two-tailed paired Student’s t-test). Unprocessed original scans of all blots with size marker are shown in Supplementary Fig. 9 Source data for e–h are in Supplementary Table 8.
