Supplementary Figure 8: Targeting MAYA inhibits the migration of lung cancer cells in vitro and tumor bone metastasis in vivo.
From: A ROR1–HER3–lncRNA signalling axis modulates the Hippo–YAP pathway to regulate bone metastasis
(a) RT-qPCR detection of MAYA expression level in a panel of breast cancer cell lines. (b) RT-qPCR detection of MAYA expression level in BoM-1833 cells harboring indicated shRNAs. (c) Cell proliferation assay of BoM-1833 cells harboring indicated shRNAs. (d and e) Cell migration (d) and invasion (e) assays using BoM-1833 cells harboring indicated shRNAs (left, Scale bars, 200 μm; right, quantification). (f) RT-qPCR detection of MAYA expression level in bone tumors of nude mice inoculated with BoM-1833 cells harboring indicated shRNAs. (g) RT-qPCR detection of MAYA expression level in A549-Luc cells harboring indicated shRNAs. (h) Cell proliferation assay of A549-Luc cells harboring indicated shRNAs. (i and j) Cell migration (i) and invasion (j) assays using A549-Luc cells harboring indicated shRNAs (left, Scale bars, 200 μm; right, quantification). (k and l) Representative BLI images (k) and bone colonization (l) of nude mice with intra-cardiac injection of A549-Luc cells harboring indicated shRNAs (n = 5 mice per group). (m) Graphic illustration of the functional role of NRG1-HER3 triggered, MAYA-mediated Hippo signaling suppression and YAP targets activation for promoting bone metastasis. For a–j and l, mean ± s.e.m. were derived from n = 3 independent experiments (∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001, two-tailed paired Student’s t-test). Statistics source data for d,e,i,j and k–l are in Supplementary Table 8.
