Supplementary Figure 7: Generation of LINK-A PIP3-binding motif knockout cell line by CRISPR/Cas9 gene editing. | Nature Cell Biology

Supplementary Figure 7: Generation of LINK-A PIP3-binding motif knockout cell line by CRISPR/Cas9 gene editing.

From: The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors

Supplementary Figure 7

(a) gRNA sequences targeting LINK-A PIP3-binding motif. (b) PCR analysis of targeted locus showing the band corresponding to the genomic deletion in colonies #1, 3 and 6. (c and d) Genotyping PCR results showing the deletion of LINK-A PIP3 binding region in colonies #3 (c) and #6 (d) of MDA-MB-231 cells. Unprocessed original scans of all gels with size marker are shown in Supplementary Fig. 9.

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