Supplementary Figure 1: Characterization of lipid-lncRNA interaction.
(a) Lipid binding affinity (Enrichment, lipid/total, L/T%) of TNBC-upregulated lncRNAs were calculated using normalized density. (b) Fold change of lncRNAs expression in tumor tissues compared to normal breast tissue. (c and d) Lipid-coated beads pull-down followed by RT–qPCR detection of LINK-A-lipid (c) or GAPDH-lipid (d) interactions. (e) Fluorescence spectra of BODIPY FL-PIP3 (donor) in the presence of Alexa-555-Strep alone (black line) or with Alexa-555-Strep-biotin-RP11.38310.5 (red line). (f) Graphic illustration of Alpha assay using DIG-LINK-A and Biotin-PIP3. (g) Competition binding Alpha assay to determine Kd for interaction between Biotin-PIP3 and DIG-LINK-A, in the presence of unlabeled full-length LINK-A (left), PC-binding motif (middle) or PIP3-binding motif (right) titrated from 0.4 mM to 0.05 nM (mean ± s.e.m. were derived from n = 3 independent experiments). For c and d, mean ± s.e.m. were derived from n = 3 independent experiments (*P < 0.05, two-tailed paired Student’s t-test).
