Supplementary Figure 2: Alignment of Kif25 protein sequences obtained by blast search of human Kif25 sequence.

Alignment was created using CLC Sequence Viewer version 7.0.2 (CLC Bio, Cambridge, MA). Macaca fascicularis Kif25 protein, 586 amino acids (GenBank Accession AB168279), Miki et al. human Kif25, 438 amino acids (Reported in Miki et al., 2005, supplemental application 3), and Okamoto et al., 1998 human Kif25, 384 amino acids (GenBank accession number NP_085118) represent isolated cDNA sequences while all other sequences are predicted based on genome analysis algorithms. Macaque Kif25, Chimp Kif25, 585 amino acids (GenBank accession SM_003820208), Finch Kif25, 587 amino acids (Genbank accession XP_002189451), and Stickleback Kif25, 580 amino acids (UniProt number G3PCB8), all show significant N-terminal extensions relative to the initial human Kif25 sequence. Human Kif25 from Miki et al. contains an additional 54 N-terminal amino acids relative to the original Okamoto et al. human Kif25 sequence suggesting that an N-terminal extension is present on human Kif25 as well. Residues in alignment colored red show <33% homology between sequences, black residues are between 33–66% homologous and blue residues are >66% homologous between sequences. Percent conservation (0–100%) of the sequences at each position is shown as a line plot below the alignment. Very high sequence homology is observed in the putative kinesin motor domain at the C-terminus of each protein.