Supplementary Figure 4: Accumulation of KBP does not induce any major transcriptional rearrangement.

mESCs were infected with lentiviruses expressing either HA-tagged wild type mouse KBP or HA-tagged mouse KBP(KK/RR) and maintained in self renewing conditions. RNA-seq analysis was performed and results are shown as a MA plot of log2 fold changes versus the mean of normalized counts. Genes were considered differentially expressed when the P adjusted value was <0.05. ∼290 genes displayed differential expression, with modest fold changes (the relative expression for each affected transcript is comprised between −0.2 and +0.3). Neither a variation in the expression of the pluripotency core factors nor changes in the major metabolic drivers were detected. The complete list of FPKM reads is reported in Supplementary Table 2. n = 3 biologically independent experiments.