Supplementary Figure 6: Inhibition of KBP degradation induces a gain in mitonchondrial mass similar to that observed during differentiation.

(a) mESCs stably infected with lentiviruses expressing either HA-tagged wild type mouse KBP or HA-tagged mouse KBP(KK/RR) were treated for 12 h with Qc-1. Cells were collected and lysed for immunoblotting as indicated. (b–c) mESCs infected with lentiviruses expressing either HA-tagged wild type mouse KBP or HA-tagged mouse KBP(KK/RR) were either maintained in LIF-containing medium or induced to differentiate for 24 h after LIF withdrawal and exposure to retinoic acid (RA). Where indicated, cells were treated for 12 h with the TDH inhibitor Qc-1. Panel (b) shows the MIPs of representative cells (scale bar, 15 μm). Panel (c) shows the quantification of average mitochondrial count. Error bars indicate ± s.d. n = 108, 17, 56, 129, 66 and 39 cells [KBP – DMSO, Qc-1 and differentiation- and KBP(KK/RR) – DMSO, Qc-1 and differentiation-, respectively]. P value was calculated by two-way ANOVA and multiple comparison. Unprocessed original scans of blots are shown Supplementary Fig. 9. Unless otherwise noted, experiments were performed at least three times.