Supplementary Figure 7: Defects in KBP degradation do not affect mitochondrial dynamics.
From: The TDH–GCN5L1–Fbxo15–KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells
(a–f) mESCs were infected with lentiviruses expressing either HA-tagged wild type mouse KBP or HA-tagged mouse KBP(KK/RR) and then transiently transfected with a plasmid expressing mitochondrial-targeted GFP. Panels (a) and (d) show representative 3D reconstructions of the mitochondria. Panels (b) and (e) show the entity of mitochondrial size changes registered in a and d, respectively. Panels (c) and (f) show the quantification of the events of mitochondrial fission and fusion. n = 6 and 7 independent field acquisitions [KBP and KBP(KK/RR), respectively]. Statistics was calculated with unpaired t-test. This experiment was performed once.
