Supplementary Figure 6: Two-color localization microscopy with protein-specific antibodies and skylan-clathrin (light chain) in intact HeLa cells.
From: Endocytic proteins are partitioned at the edge of the clathrin lattice in mammalian cells
A gallery of example images for clathrin, dynamin2, AP2, epsin1, EPS15, and DAB2 are shown. Skylan-clathrin is shown in green, and antibody staining is shown in magenta (specific antibodies are described in methods, primary antibodies were supplemented with AF647-labeled secondary antibodies). Note the increased background fluorescence in intact cells. Scale bar, 400 nm. Two independent images were processed for each except for clathrin (N = 3) and DAB2 (N = 1).
