Supplementary Figure 1: NC contact the dorsal aorta prior to and during HSC specification. | Nature Cell Biology

Supplementary Figure 1: NC contact the dorsal aorta prior to and during HSC specification.

From: Pdgf signalling guides neural crest contribution to the haematopoietic stem cell specification niche

Supplementary Figure 1

Trunk lateral views (a–f; dorsal up, anterior left) of NC migration progress using the NC reporter sox10:mRFP relative to endothelial cells labeled with EGFP in fli1a:EGFP animals. Images are still frames from a z-stack volume projection in single and merged channels (a–f) from time lapse confocal microscopy from 20 h.p.f. to 27 h.p.f. as indicated. Arrowheads indicate NC streams that are positioned ventral to (yellow arrowheads) or dorsal to (blue arrowheads) the notochord. Scale bar = 100 μm, mag. = 200X. isv – intersegmental vessel, da – dorsal aorta, pcv – posterior cardinal vein.

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