Supplementary Figure 3: Characterisation of differentiaton markers in KPC vs. KPCZ tumours. | Nature Cell Biology

Supplementary Figure 3: Characterisation of differentiaton markers in KPC vs. KPCZ tumours.

From: The EMT-activator Zeb1 is a key factor for cell plasticity and promotes metastasis in pancreatic cancer

Supplementary Figure 3

(a) Representative images of positive and negative immunohistochemical stainings and statistical analysis for the indicated EMT-TFs. Scale bar, 150 μm. n = 14 independent tumours for KPC, 13 independent tumours for KPCZ, Chi-square test (two-tailed); n.s. = not significant. (b) Representative images of immunohistochemical stainings and statistical analysis for expression of Gata6. Scale bar, 150 μm. n = 14 independent tumours for KPC, 13 independent tumours for KPCZ; error bars show mean ± S.D.; Mann-Whitney test (two-tailed), ∗∗∗p < 0.001. (c) Representative images of differentiated KPCZ and undifferentiated KPC primary tumours (PT) and corresponding metastases (Met) with the same phenotype. Immunohistochemical labelling of Zeb1 expressing tumour cells in the KPC PT and Met (arrows). L = liver or lung tissue. n = 19 KPC, 4 KPCZ independent tumours and corresponding metastases. Scale bar, 100 μm.

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