Supplementary Figure 7: Depletion of Zeb1 reduces tumour cell plasticity.

(a) Relative mRNA expression levels (qRT-PCR) of indicated genes in KPC and KPCZ cell lines treated for different times with TGFβ (time points: 0, 6 h, 1, 3, 7, 14, 21 days). mRNA levels of cell line 661 at day 0 were set to 1. n = 3 biologically independent experiments, error bars show mean ± S.E.M. Statistical analysis is shown for the comparison of TGFβ treated to untreated samples (grey bars) of each individual cell line ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, unpaired Student’s t-test (one-tailed) Source data see Supplementary Table 5, Statistics Source Data. (b) Table showing log2FC in mRNA expression levels (microarray) of genes previously determined as common ZEB1/YAP targets in KPC and KPCZ cell lines upon TGFβ treatment for 14 days. (cut-off: adj. p-value < 0.05 and log₂FC > 0.5). (c) Representative images of consecutive sections of immunohistochemistry for Ck19 and Zeb1 comparing the plasticity of Zeb1 expression in central and invasive tumour regions. Tumours derived from one KPC and one KPCZ cell line are shown. Asterisks label Zeb1 expression in stroma cells, arrows indicate Zeb1 expression in tumour cells at the invasive front. Ck19 expression is shown to identify cancer cells. n = 15 KPC, 13 KPCZ independent tumours, Scale bars, 50 μm and 150 μm for higher magnifications.