Supplementary Figure 1: Characterisation of KPC, heterozygously and homozygously Zeb1 depleted KPC tumours. | Nature Cell Biology

Supplementary Figure 1: Characterisation of KPC, heterozygously and homozygously Zeb1 depleted KPC tumours.

From: The EMT-activator Zeb1 is a key factor for cell plasticity and promotes metastasis in pancreatic cancer

Supplementary Figure 1

(a) Representative Zeb1-immunolabeling of a GFP lineage-traced primary tumour showing Zeb1/GFP double-positive tumour cells (arrows). n = 5 independent tumors. Scale bar, 50 μm. (b) Representative consecutive sections of HE and indicated immunohistochemical stainings of four Zeb1 expressing KPC tumours demonstrating the heterogeneity in phenotype, grading and marker expression. A representative differentiated Zeb1-negative KPCZ tumour is shown for comparison. Arrows indicate Zeb1 positive tumour cells in the differentiated KPC tumour. n = 15 KPC, 13 KPCZ independent tumours. Scale bar, 100 μm. (c) Tumour-free survival of KPC mice vs. KPC mice with a heterozygous deletion of Zeb1 (KPCz) (n = 15 KPC, 16 KPCz independent tumours); log-rank (Mantel-Cox) test); tumour volume (0 = start of MRI measurements, n = 12 KPC, 14 KPCz independent tumours); error bars show mean ± S.E.M.; multiple t-tests with correction for multiple comparison using the Holm-Sidak method; grading, local invasion and relative ECM deposition of the respective tumours (n = 31 KPC, 17 KPCz; Mann-Whitney test (two-tailed); percentage of metastasized tumours (n = 35 KPC, 17 KPCz independent tumours; Chi-square test (two-tailed); n.s. = not significant.

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