Supplementary Figure 4: SWELL1 is required for lipogenesis and adipocyte glucose metabolism, Related to Fig. 4.
From: SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis
(a) Heat map displaying 2-way hierarchical clustering and mRNA expression levels of WT (n = 3) and CRISPR–cas9 SWELL1 KO (n = 3) 3T3-F442A adipocytes. Columns WT 1-3 and KO 1-3 represent RNA from individual experiments. (b) Gene Set Enrichment Analysis revealing pathways and processes negatively enriched in SWELL1 KO compared with WT 3T3-F442A adipocytes. Q value is the false discovery rate (FDR)-adjusted p value. Q < 0.10 is considered statistically significant. (c) Representative fluorescence images of AdipoRed stained WT and SWELL1 KO 3T3-F442A adipocytes under low glucose (5.6 mM) and high glucose (25 mM) culture conditions. Mean AdipoRed fluorescence intensity per cell under each condition (nWT,5.6 mM = 444, nWT,25 mM = 422, nKO,5.6 mM = 527, nKO,25 mM = 589). Measurements pooled from 3 separate independent experiments. Scale bar: 200 μm. (d) Transmission Electron Microscopy (TEM) images of glycogen granules in WT and SWELL1 KO adipocytes. Scale bar: 0.5 μm. Significance between the indicated groups in c were calculated using a two-tailed Student’s t-test. Exact P-values are listed in Supplementary Table 6. Error bars represent mean ± s.e.m.∗∗∗(P < 0.001).
