Supplementary Figure 7: WT and Adipo KO adipocyte size, expression of adipocyte differentiation genes and indirect calorimetry data in lean, regular chow fed mice, Related to Fig. 7.
From: SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis
(a) Bright field images of mature adipocytes isolated from iWAT of lean littermate WT and Adipo KO mice. Scale bar: 50 μm. (bc) Mean adipocyte area (b) and size distribution (c) of WT (n = 1214) and Adipo KO (n = 982) adipocytes. Measurements pooled from 3 pairs of mice. (d) H&E staining of iWAT adipose tissues in lean littermate WT and Adipo KO mice. Scale bar: 50 μm. (e,f) Mean adipocyte cross-sectional area (e) and size distribution (f) of WT (n = 979) and Adipo KO (n = 1118) adipocytes. Measurements pooled from 3 pairs of mice. (g) Relative mRNA expression of adipocyte differentiation markers assessed by qPCR (nWT = 8, nAdipoKO = 5). (h-n) Metabolic cage studies were performed on WT (n = 9) and Adipo KO (n = 7) mice fed a regular chow diet (13–19 weeks of age). The mice were housed individually in the chambers for 5 days, and data was analyzed from the last 3 days. (h) Heat production; (i) Respiratory exchange ratio (RER); (j) CO2 production; (k) O2 consumption; (l) Physical activity; (m) Food consumption; and (n) Sleep. Significance between the indicated groups were calculated using a two-tailed Student’s t-test. Exact P-values are listed in Supplementary Table 6. Error bars represent mean ± s.e.m.∗(P < 0.05).
