Supplementary Figure 4: TFAM and PHB2 are required for human erythropoiesis.

(a) Erythroid maturation was assessed by the expression of CD71 and CD235a in cells transduced with shRNAs targeting TFAM, PHB2, or control (shNT). (b) Example cytospin preparations from uninfected, control (shNT), TFAM, or PHB2-depleted cells stained with May-Grunwald-Giemsa. Scale bars, 20 μm. The images represent one out of three independent experiments. (c) Expression of hemoglobin genes (HBA1 and HBB), GATA1 and GATA2 was assessed using qRT-PCR. Results are mean ± s.e.m. of n = 3 independent experiments. Differences relative to shNT were assessed using a repeated-measures one-way ANOVA followed by Dunnett’s test for multiple comparisons. ^∗∗P < 0.01, ^∗∗∗P < 0.001. See Statistics Source Data in Supplementary Table 8. (d,e) Gene set enrichment analysis (GSEA) of gene signatures associated with apoptotic signaling pathways using the RNA-seq data of shTFAM or shPHB2 relative to shNT, respectively. The normalized enrichment score (NES) and nominal P-value are shown.