Supplementary Figure 1: Differentially expressed transcripts and proteins in human primary fetal and adult HSPCs and ProEs.

(a,c,e,g) Scatter plot of transcriptomic profiling between the indicated adult CD34⁺ HSPCs (A0), fetal CD34⁺ HSPCs (F0), adult ProEs (A5), and fetal ProEs (F5) is shown. The numbers of significantly upregulated or downregulated transcripts (fold change ≥1.5, P-value ≤ 0.01) are indicated. (b,d,f,h) Gene ontology (GO) analysis of transcripts upregulated in the indicated adult HSPCs (A0), fetal HSPCs (F0), adult ProEs (A5) or fetal ProEs (F5) was performed using DAVID tools. (i,k,m,o) Scatter plot of proteomic profiling between the indicated adult CD34⁺ HSPCs (A0), fetal CD34⁺ HSPCs (F0), adult ProEs (A5), and fetal ProEs (F5) is shown. The numbers of significantly upregulated or downregulated proteins (fold change ≥1.3, P-value ≤ 0.01) are indicated. (j,l,n,p) GO analysis of proteins upregulated in the indicated adult HSPCs (A0), fetal HSPCs (F0), adult ProEs (A5) or fetal ProEs (F5) was performed using DAVID tools. (r) The correlation between RNA and protein expression changes in adult HSPCs (A0) and ProEs (A5) is shown. Differentially expressed RNAs and proteins (red, upregulated; blue, downregulated) were accessed for overlap. After filtering genes detected exclusively by RNA-seq or proteomics, 2,160 and 785 significant downregulated RNAs (RNA-all) and proteins (Protein-all) were identified, respectively. The differentially expressed genes were further divided into three groups based on significant changes at RNA (RNA-only), protein (Protein-only), and both levels, respectively. (s) Similar to r, the correlation between RNA and protein expression changes in fetal HSPCs (F0) and ProEs (F5) is shown. Differentially expressed RNAs and proteins (dark green, upregulated; light green, downregulated) were accessed for overlap. After filtering genes detected exclusively by RNA-seq or proteomics, 987 and 976 significant upregulated RNAs (RNA-all) and proteins (Protein-all) were identified, respectively. The differentially expressed genes were further divided into three groups based on significant changes at RNA (RNA-only), protein (Protein-only), and both levels, respectively. (t) The correlation between downregulated RNA transcripts and proteins in fetal HSPCs (F0) and ProEs (F5) is shown.