Supplementary Figure 4: Bone marrow mesenchymal stromal cells down-regulate CXCL12 and SCF, and display a fibrotic cell morphology. (Related to Fig. 4).
From: Leptin-receptor-expressing bone marrow stromal cells are myofibroblasts in primary myelofibrosis
(a,b) Representative images showing normal Cxcl12-DsRed expression in Cxcl12DsRed/+ mice transplanted with control virus-infected bone marrow cells. (c,d) Images showing Cxcl12-DsRed expression in TOE mice. Arrow heads point to elongated fibroblast-like stromal cells. (e) qPCR analysis showing downregulation of Scf and Cxcl12 in bone marrow mesenchymal stromal cells from intermediate stage to advanced stage PMF mice (n = 4 mice for control, n = 4 mice for intermediate PMF and n = 4 mice for advanced PMF). Data represent mean ± s.d. Two-tailed student’s t-tests were used to assess statistical significance. (f) Representative flow cytometry plots showing CD45/Ter119−Scf-GFP+ stromal cells. Images are representative of at least 3 biological replicates.
