Supplementary Figure 1: Substrate specificity of ubiquitin receptors.
From: Receptor oligomerization guides pathway choice between proteasomal and autophagic degradation
a, Degradation of Ub-βgal-WT in WT and mutant cells. Degradation is strongly impaired in cells deficient in Dsk2, mildly impaired in cells lacking the Dsk2-related protein Rad23, but efficiently degraded in cells lacking the autophagy proteins Cue5 or Atg8. Experiment was performed as in Fig. 1e. Representative image of two independent replicates is shown. b, Cue5 binds the aggregation-prone substrate variant Ub-βgal-X90 stronger than Ub-βgal-WT. Binding does not occur when the ubiquitin-binding CUE is altered (CUEmut). Experiment was performed as in Fig. 3a. Representative image of two independent replicates is shown. c, In contrast to Dsk2-GFP, Cue5-GFP co-localized extensively with TDP-43-mars aggregates (scale bar, 5 μm). Wide-field of cells similar to Fig. 1i. Representative image of two independent replicates is shown. Unprocessed original scans of blots are shown in Supplementary Fig. 5.
