Supplementary Figure 1: ACAT2 is stabilized by sterols and fatty acids.
From: Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation
(a) Structures of different sterols. Red denotes sterols that elevated ACAT2 protein level. (b) CHO/ACAT2-Myc cells were depleted of lipids and treated with different sterols at indicated concentration. 16 hrs later, cells were harvested for western blotting. (c,d) Different cell lines (HepG2, Huh7, Hepa1-6 and Caco2) were depleted of lipids and treated with OA (200 μM) or PA (200 μM). 16 hrs later, cells were harvested for western blotting and RT-qPCR (mean ± s.d., n = 3 independent experiments). The immunoblots are representative of 3 independent experiments. Uncropped blots are shown in Supplementary Fig. 8. Statistics source data for d can be found in Supplementary Table 2.
