Supplementary Figure 2: Endothelial VEGF signalling in Notch-dependent angiogenesis.
From: Dll4 and Notch signalling couples sprouting angiogenesis and artery formation
(a,b) Representative overview (a) and confocal images (b) of IB4-stained VegfaiΔEC/+ and control retinas treated with vehicle or with DAPT. Circles indicate vessel outgrowth in control. (c) Representative overview pictures of IB4-stained VegfaiΔEC/ΔEC and control retinas treated with vehicle or with DAPT. Circles indicate vessel outgrowth in control. (d,e) Confocal images of IB4 (green), ESM1 (blue) and ERG (red) stained vehicle (d) and DAPT-treated (e) VegfaiΔEC/ΔEC and control P6 retinas. Note residual ESM1 staining (arrowheads) in VegfaiΔEC/ΔEC EC sprouts but impaired DAPT-induced upregulation in the plexus (arrows) relative to control. (f) Confocal images of IB4 (green), VEGF-A (blue) and CXCR4 (red) staining of P6 retina. Note correlation (arrowheads) between CXCR4 and VEGF-A immunosignals.
