Supplementary Figure 4: Role of CXCR4 in Notch-controlled angiogenesis.
From: Dll4 and Notch signalling couples sprouting angiogenesis and artery formation
(a) Representative overview images showing DAPT-treated Cxcr4iΔEC/ΔEC and control retinas. Circles indicate vessel outgrowth in control. (b) Confocal images showing vehicle- or DAPT-treated Cxcr4iΔEC/ΔEC and control retinas. Note the decrease sprouting (arrowheads) in DAPT-treated Cxcr4iΔEC/ΔEC compared to control littermates. (c) Overview images of IB4-stained untreated or AMD3100-treated Dll4iΔEC/ΔEC retinas. Circles indicate vessel outgrowth in control (d) (e) Representative overview pictures. (d) and high magnification confocal images (e) of IB4-stained Cxcr4iΔEC/KO RbpjiΔEC/ΔEC and Cxcr4iΔEC/+ RbpjiΔEC/ΔEC retinas. Sprouting (arrowheads) was reduced in Cxcr4iΔEC/KO RbpjiΔEC/ΔEC retinas compared to Cxcr4iΔEC/+ RbpjiΔEC/ΔEC control retinas. (f) Representative confocal images of IB4-stained Cxcr4iΔEC/ΔEC Dll4iΔEC/ΔEC and Dll4iΔEC/ΔEC retinas. Sprouting (arrowheads) was reduced in Cxcr4iΔEC/ΔEC Dll4iΔEC/ΔEC retinas relative to Dll4iΔEC/ΔEC control retinas. (g) Quantitative analysis of vascular parameters for Cxcr4iΔEC/ΔEC Dll4iΔEC/ΔEC, Dll4iΔEC/ΔEC and control (Cre negative) retinas: retinal vessel progression, EC area and sprouting (n = 8 control, 6 Dll4iΔEC/ΔEC and 6 Cxcr4iΔEC/ΔEC Dll4iΔEC/ΔEC retinas). Note significant differences in sprouting but not in vessel progression and EC area between Cxcr4iΔEC/ΔEC Dll4iΔEC/ΔEC and Dll4iΔEC/ΔEC retinas. Data represent mean ± s.e.m. P values, one-way ANOVA with Tukey’s multiple comparison post-hoc test.
