Supplementary Figure 2: Genome editing strategies for disruption of CTCF binding at the α-globin locus. | Nature Cell Biology

Supplementary Figure 2: Genome editing strategies for disruption of CTCF binding at the α-globin locus.

From: Tissue-specific CTCF–cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo

Supplementary Figure 2

Schematic overview of the mutagenesis method (CRISPR-Cas9 and/or TALEN) and the resulting mutations induced at HS-38 (D38), HS-39 (D39), HS-38 and HS-39 (D3839), and HS-29 (D29). DNaseI footprints are annotated with matches to the C, U, and D CTCF motif (coloured bar under C, red = forward, blue = reverse). Where homology directed repair was used to replace part of the CTCF core motif with a restriction site, the single-stranded oligodeoxynucleotides (ssODNs) used as repair templates are shown.

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