Supplementary Figure 2: Validation of hair cycle stage.
From: Lactate dehydrogenase activity drives hair follicle stem cell activation
a, Analysis of RNA-seq data to validate that HFSCs in telogen-anagen transition were in fact in such a transition. The telogen-anagen transition is known to be driven by Shh (Gli factors are targets) and Wnt (Lef1, Axin, Ccnd1 are targets) signaling, and correlate with increased proliferation (Ki-67 and Pcna). In addition, Sox4 was previously identified as a regulator of the telogen-anagen transition. n = 3 mice per timepoint. Shown as mean ± s.e.m. Paired t-test was performed, P < 0.05. b, staining for Ki-67 marks dividing cells during various stages of the hair cycle. Brackets indicate the HFSC niche. Scale bars indicate 100 micrometers.
