Supplementary Figure 1: ARID1A-inactivated cells are more sensitive to HDAC6 inhibition.

(a) ARID1A wild-type RMG1 cells with or without ARID1A knockdown were transduced with lentivirus encoding shRNA to each of the 11 individual HDACs. RNA was isolated from the indicated cells and subjected to qRT-PCR for the indicated HDACs. n = 3 independent experiments. (b) Confirmation of HDAC6 knockdown by qRT-PCR in OVCA429 cells. n = 6 independent experiments. (c) HDAC6 knockdown is selective against ARID1A-mutated clear cell or endometrioid ovarian cancer cell lines based on the Project Achilles database. The whiskers of the boxplot represent minima to maxima of the relative growth of cell lines with (n = 5 cell lines) or without ARID1A mutation (n = 11 cell lines). The box represents median bar with the first and the third quartiles. (d) Expression of HDAC6 determined by immunoblot in a panel of ovarian clear cell carcinoma cell lines with known ARID1A mutational status without or with HDAC6 knockdown using two individual shHDAC6s. GAPDH expression was used as a loading control. Error bars represent mean with S.E.M. P-value calculated via two-tailed t-test. Statistical source data are provided in Supplementary Table 6. Unprocessed original scans of all blots with size marker are shown in Supplementary Fig. 9.