Figure 1: The NFI-binding motif is highly enriched in brown-fat-specific open chromatin regions.
From: NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program
(a) Representative FAIRE-seq tracks of murine BAT, iWAT and eWAT. (b) Venn diagram showing overlap of BAT, iWAT and eWAT FAIRE peaks. (c) Motifs enriched in BAT-specific FAIRE peaks and eWAT- or iWAT-specific FAIRE peaks. The motif analysis was performed once on the basis of the FAIRE-seq data set. (d) mRNA expression levels of the NFI family in C2C12 myoblasts, immortalized brown adipocytes and 3T3-L1 white adipocytes before (pre) and after (diff) differentiation (mean ± s.e.m.; n = 3 independent samples). (e) mRNA expression levels of the NFI family in soleus muscle, quadriceps muscle, BAT, iWAT and eWAT (mean ± s.e.m.; n = 7 independent samples; ∗P < 0.05, ∗∗P < 0.01). (f) Western blot analysis of NFIA in representative samples from soleus muscle, quadriceps muscle, BAT, iWAT and eWAT. β-actin was used as a loading control. Representative images of two independent experiments are shown. Unprocessed original scans of blots are shown in Supplementary Fig. 8.
