Figure 4: NFI binding is enriched at brown-fat-specific enhancers.
From: NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program
(a) Genomic location of NFI-binding sites in brown adipocytes at day 0 and day 6 of differentiation. (b) Enriched known motifs within NFI-binding sites in brown adipocytes at day 6 of differentiation. (c) Representative tracks of ChIP-seq for NFI, PPARγ, C/EBPα, C/EBPβ, EBF2 and H3K27Ac in brown adipocytes, ATAC-seq of brown adipocytes, FAIRE-seq of in vivo BAT, and ChIP-seq for NFIA in NFIA-expressing C2C12 myoblasts at Pparg, Cidea and Ucp1 loci. (d) The distance from the TSS to the nearest NFI-binding site for BAT- and WAT-selective genes. The definitions of BAT- and WAT-selective genes (n = 549 and n = 849, respectively) are shown in the Methods. (e) Number of NFI-binding sites within ±50 kb of the TSS for BAT- and WAT-selective genes (n = 549 and n = 849, respectively). (f) Box plot showing the strength of the NFI binding signal (MACS score) near all genes (n = 21,258), BAT- and WAT-selective genes (n = 549 and n = 849, respectively). The box shows the median, and the first and third quartiles. The whisker shows the value still within one-and-a-half times the interquartile range. The genome-wide analyses were performed once on the basis of the ChIP-seq data set.
