Supplementary Figure 1: Additional images of flow gating and IHC in mouse and human. | Nature Cell Biology

Supplementary Figure 1: Additional images of flow gating and IHC in mouse and human.

From: Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism

Supplementary Figure 1

(a) Gating startegy for sorting of Lgr5-EGFP positive stem cells for transcript abundance. Top showing final gating for collection of GFP-low, GFP-mid, and GFP-high cells for transcript abundance. Bottom showing full gating strategy exclusion of cell debris and purification of singlet cells. (b) IHC of VDAC1 and MPC1 throughout the mouse small intestine from proximal to distal showing staining of crypt base and differentiating transient amplifying cells. (scale bar = 20 μm). (c) Human Protein Atlas (http://www.proteinatlas.org) images of duodenum, small intestine, and colon stained for TFAM, NRF1, PDK1, and CK20 (scale bar = 20 μm (b), 100 μm (c)).

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